[Clinical comparison of mechanical and chemomechanical methods in removing deep dentinal caries]

The use of Carisolv[TM] decreases unnecessary removal of sound dental tissue and reduces the possibility of pulpal exposure. However, it is expensive and time-consuming and its efficacy has not been clearly " defined. The aim of this study was to compare the efficacy, safety and efficiency of removing deep caries using conventional mechanical methods alone and in conjunction with Carisolv. This investigation was a single-blind randomized clinical trial. Forty individuals, each with a deep carious tooth [0.5-1 mm from the pulp] without signs and symptoms of necrosis or irreversible pulpitis were selected and randomly divided into two study-groups: A, all caries were mechanically removed using rotary instruments and B, Carisolv was applied on deep carious lesions and the rest of the caries were mechanically eliminated. All cavities were evaluated by a dental probe. After complete caries removal the teeth were filled as usual. The outcome variables were complete caries removal, pulpal exposure and time taken to remove caries. The results were analyzed by X[2] and Mann-Whitney tests. Three pulpal exposures occurred in group A and one in group B without a statistically significant difference. Regarding complete caries removal, the two methods were similar. The mean caries-removal time was 16 +/- 6.8 min in group A and 25.2 +/- 6.3 min in group B [P<0.001]. The amount of Carislov used for each tooth was 0.17 cc. Application of the mechanical and chemomechanical methods simultaneously, is more time-consuming and does not seem to significantly improve the treatment results

Liberación estriatal de un análogo de aminoácidos excitatorios en un modelo experimental de hipoxia cerebral in vitro / Striatal release of a tritiated analog of excitatory amino acid in a in vitro experimental model of brain hypoxia

Este trabajo presenta la metodología experimental de un modelo de hipoxia cerebral in vitro. El modelo fue empleado para evaluar la liberación de un análogo no metabolizable de glutamato en condiciones de hipoxia, como un reflejo del estado funcional de los terminales nerviosos glutamatérgicos en condiciones de hipoxia, observándose una significativa liberación del marcador tritiado en relación a la hipoxia, lo cual concuerda con hallazgos previos tanto en animales de experimentación como en seres humanos. La metodología presentada es simple y permite manejar a voluntad del investigador una serie de parámetros relevantes desde el punto de vista fisiopatológico de la injuria neuronal por hipoxia, de un modo que es imposible de lograr en los estudios in vivo